The image shows a nucleus extracted from the brain of an old (29 month) rat and immuno-stained for tubulin (green)and nuclear pore complexes (red). DNA was stained with DAPI (blue). The intra-nuclear...

3HA-Cdc14 (red) localization in early anaphase. The protein is partially released throughout the nucleus. Early anaphase is determined by spindle morphology (tubulin, green) and nuclear morphology (DA...

3HA-Bfa1 (red) localizes to both spindle pole bodies (SPBs) in anaphase in dyn1Δ cells with constitutive targeting of Tem1 to both SPBs. However, the constitutive presence of Tem1 on the SPBs impaire...

Cdc15-3HA (red) does not load onto spindle pole bodies in wild-type cells in metaphase as determined by spindle morphology (tubulin, green) and nuclear morphology (DAPI, blue). Control image for CIL# ...

3HA-Cdc14 (red) localization in anaphase. The protein is released throughout the whole cell. Anaphase is determined by spindle morphology (tubulin, green) and nuclear morphology (DAPI, blue). Image is...

3HA-Bfa1 (red) is generally localized to one spindle pole body (SPB) in wild-type cells in metaphase as determined by spindle morphology (tubulin, green) and nuclear morphology (DAPI, blue). Control i...

3HA-Bfa1 (red) is generally localized to the daughter spindle pole body (SPB) in wild-type cells in anaphase as determined by spindle morphology (tubulin, green) and nuclear morphology (DAPI, blue). C...

3HA-Bfa1 (red) is now symmetrically localized to both spindle pole bodies (SPB) in cells with constitutive targeting of Tem1 to both SPBs in metaphase as determined by spindle morphology (tubulin, gre...

3HA-Bfa1 (red) is now symmetrically localized to both spindle pole bodies (SPB) in cells with constitutive targeting of Tem1 to both SPBs in anaphase as determined by spindle morphology (tubulin, gree...

In tem1Δ cells expressing a Cnm67-Tem1 fusion protein, Cdc15-3HA (red) loads prematurely onto spindle pole bodies during metaphase as determined by spindle morphology (tubulin, green) and nuclear mor...